DNA

Part:BBa_K289011:Design

Designed by: Nan Hou   Group: iGEM09_Tianjin   (2009-10-21)


cyano-yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 21
    Illegal BamHI site found at 3072
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
    Illegal AgeI site found at 1516
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the pSBA1 promoter is from the cyanobacteria Synechococcus sp.PCC 7942, since promoters from cyanobacteria is more efficient than foreign promoters in Anabaena sp.PCC 7120


Source

complete synthesis

References